Dominant Selectable Markers for Penicillium spp. Transformation and Gene Function Studies

Selectable Markers for Gene Therapy

One of the major problems with current approaches to gene 9 therapy is the instability of expression of genes transferred into 10 recipient cells. Although in theory, homologous recombination 11 or use of artificial chromosomes can stabilize sequences with 12 wild-type regulatory regions, such approaches to gene therapy 13 are not yet feasible and may not be efficient for some time to 14 come. ...

Comparison of two alternative dominant selectable markers for wine yeast transformation.

Genetic improvement of industrial yeast strains is restricted by the availability of selectable transformation markers. Antibiotic resistance markers have to be avoided for public health reasons, while auxotrophy markers are generally not useful for wine yeast strain transformation because most industrial Saccharomyces cerevisiae strains are prototrophic. For this work, we performed a comparati...

The chloroplast transformation toolbox: selectable markers and marker removal.

Plastid transformation is widely used in basic research and for biotechnological applications. Initially developed in Chlamydomonas and tobacco, it is now feasible in a broad range of species. Selection of transgenic lines where all copies of the polyploid plastid genome are transformed requires efficient markers. A number of traits have been used for selection such as photoautotrophy, resistan...

Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers.

Genetic manipulation using linear DNA was applied to the common wheat variety Xindong No. 26 via particle bombardment with the aim to improve bread-making quality of flour. Initially, 2 biolistic parameters, helium pressure and target distance, were optimized using plasmid pAHC25. We transformed wheat immature embryo scutella with the linear 1Dx5 gene without selectable markers. The highest tra...

Cloning and Mutagenesis of the for Resistance to Pseudomonic Acid by Generation of Dominant Selectable Markers